Resources

Nature Communications 2015 Supplementary Data Files

Riabinina, O, Task, D, Marr, E, Lin, C-C, Alford R, O’Brochta, DA, Potter, CJ.  Organization of Olfactory Centers in the Malaria Mosquito Anopheles gambiae. Nature Communications. 2016;7,1–12.  Link

Including 3D models of the Anopheles antennal lobe.

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Source data files

• Source Data 1  Counts of GFP+ cells in adult antennae, maxillary palp, and labellar tissues in males and females. Excel spreadsheet.
• Source Data 2  Quantification of anti-ORCO and anti-CD8 staining in labella. Excel spreadsheet.
• Source Data 3  Counts of anti-ORCO and GFP+ cells in sections of adult female antennae. Excel spreadsheet.
• Source Data 4  Quantification of adult male and female brain and antennal lobe volumes. Excel spreadsheet.
• Source Data 5  Confocal sections of female antennal lobe with glomeruli outlined. Source confocal data for Fig. 4a.  .tif file (442.4 mb)

Models

Recommended free viewers include freeWRL or Cortona 3D Viewer.

• Model S1  Antennal Lobe 3D Model, transparent (3D .wrl file, 210.5 mb). Related to Fig. 4a.
• Model S2  3D Model of adult SEZ with nerve bundles (3D .wrl file, 22 mb). Related to Fig. 4d.
• Model S3  3D Model of proboscis backfill in the SEZ.  (3D .wrl file, 22 mb). Related to Fig. 4d.

High Resolution Supplemental Figures

(from Supplemental Information)

• Supplementary Figure 1. DNA constructs for Anopheles gambiae
• Supplementary Figure 2. Background expression pattern of QUAS-CD8:GFP transgenic animals.
• Supplementary Figure 3. Orco-QF2 expression in olfactory neurons in larva.
• Supplementary Figure 4. GFP fluorescence in adult olfactory tissues.
• Supplementary Figure 5. Quantification of larval antenna and maxillary palp neurons expressing Orco-QF2.
• Supplementary Figure 6. Orco-QF2 drives GFP reporter expression in larval Orco+ neurons.
• Supplementary Figure 7. Sections of adult female antenna show co-localization between GFP reporter expression and anti-Orco staining.