Resources
Q-system
The “Q-system” is a versatile repressible binary expression system that works effectively in a variety of organisms (e.g., worms, flies, plants, and mosquitoes). It consists of three main components: the QF transcription factor, the QS suppressor of QF, and a QUAS-effector
Primary Papers
QF: *Potter CJ, *Tasic B, Russler EV, Liang L, Luo L. The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Cell. 2010;141(3):536-548; *denotes equal contribution Link
QF2, QF2w: Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ. Improved and Expanded Q-System Reagents for Genetic Manipulations. Nature Methods. 2015;12(3):219-222. Link
Reviews
Riabinina, O, Potter, CJ. “The Q-system: A Versatile Expression System for Drosophila.” Drosophila: Methods and Protocols, Second Edition. Ed. C. Dahmann. New York: Humana Press, 2016. 53-78. Link
Potter CJ, Luo L. Using the Q System in Drosophila Melanogaster. Nature Protocols. 2011;6(8):1105-1120. Link
Q-system DNA plasmids at Addgene:
Potter Lab plasmids | Q-system plasmids from Potter, 2010
We included full GenBank files with our submissions to Addgene.
Q-system fly stocks at Bloomington:
Recommended Plasmids
Drosophila
- QF2: pattB-QF2-hsp70 | pattB-nsyb-QF2-hsp70
- QF2w: pattB-nsyb-QF2w-hsp70
- QUAS: pQUAST (5x QUAS) | pattB-QUAST (5xQUAS)
- QS: pAC-QSco | pCasper4-tubulinP-QSco-p10
Mosquito
- QF2: piggyBac-QF2-hsp70-3xP-RFP | pBac-ORCO_9kbProm-QF2-3xP-RFP
- QUAS: piggyBac-15xQUAS-hsp70P-SV40T-3xP-ECFP | pBac-15xQUAS-hsp70P-mCD8:GFP-SV40T-3xP-ECFP
(Note: QF2 was originally named QF#7 in the experiments during its development. Similarly, QF2w was originally named QF#7m1. The Addgene links above take you to the correct plasmid.)