Q-System reagents - flies

QUAS reporters

Name Chromosome Bloomington Stock #
QUAS-mCD8-GFP X 30001
QUAS-mCD8-GFP #5J 2nd (23B1) 30002
QUAS-mCD8-GFP #5B 3rd (86E13) 30003
   
QUAS-mtdTomato-3xHA X (4F4) 30118
QUAS-mtdTomato-3xHA #14 2nd (49C3) 30004
QUAS-mtdTomato-3xHA #26 3rd (94E7) 30005
   
QUAS-nucLacZ X Available Upon Request
QUAS-nucLacZ #7 2nd 30006
QUAS-nucLacZ #44 3rd 30007
 
QUAS-FLPo #1 2nd (50E6) 30126
QUAS-FLPo #7 3rd (72D1) 30127
QUAS-FLPo 3rd (attP2)(68A4) 30008
   
QUAS>stop>mCD8-GFP #10 2nd (50B3) 30134
QUAS>stop>mCD8-GFP #5 3rd (93D3) 30136
QUAS>stop>mCD8-GFP #9 3rd (68A1) 30135
   
QUAS-shibirets1 #2 X (3A6) 30010
QUAS-shibirets1 #3 X (3B6) 30011
QUAS-shibirets1 #8 2nd (25B1) 30013
QUAS-shibirets1 #5 3rd (88E5) 30012
   
QUAS>stop>shibirets1 #16 3rd (82E7) 30128
   
QUAS-dmyc #7 2nd 30009
   
QUAS-GAL80 X, 2nd, 3rd Available Upon Request
   
QUAS-Rab3-GFP 2nd (attP40; 25C6) 36352 (Stowers, 2011)
QUAS-Rab3-GFP 3rd (VK00027; 89E11) 36363 (Stowers, 2011)
   
QUAS-Rab3-mCherry 2nd (attP40; 25C6) 36353 (Stowers, 2011)
QUAS-Rab3-mCherry 3rd (VK00027; 89E11) 36364 (Stowers, 2011)
   
QUAS-eNpHR3.0-YFP (halorhodopsin) 2nd (attP33; 50B6) 36355 (Stowers, 2011)
QUAS-eNpHR3.0-YFP (halorhodopsin) 3rd (attP2; 68A4) 36366 (Stowers, 2011)
   
QUAS-ChETA-YFP (ultrafast channelrhodopsin-2) 2nd (attP33; 50B6) 36356 (Stowers, 2011)
QUAS-ChETA-YFP (ultrafast channelrhodopsin-2) 3rd (attP2; 68A4) 36367 (Stowers, 2011)

QF lines

Name Chromosome Bloomington Stock #
GH146-QF #11 2nd (49E1) 30014
GH146-QF #53 3rd (68D1) 30015
   
ET6-QF 3rd (68D1) 30016
ET9-QF 2nd (49E7) 30017
ET31-QF 2nd (52E11) 30018
ET40-QF 2nd (49E6) 30019
ET49-QF 3rd (70C15) 30020
   
synaptojamin-QF 2nd (attP40; 25C6) 36357 (Stowers, 2011)
   
Tyramine-beta-hydroxylase-QF 3rd (attPVK00013; 76A1) 36365 (Stowers, 2011)
   
trpl-QF 3rd (attPVK00027; 89E11) 36370 (Stowers, 2011)
   
iav-QF 3rd (attP154; 97D2) 36347 (Stowers, 2011)
   
nompC-QF 2nd (attP40; 25C6) 36349 (Stowers, 2011)
nompC-QF 3rd (attP154; 97D2) 36346 (Stowers, 2011)
   
TrpA1-QF 2nd (attP40; 25C6) 36348 (Stowers, 2011)
TrpA1-QF 3rd (attP154; 97D2) 36345 (Stowers, 2011)
   
n-synaptobrevin-QF2 3rd (attP2; 68A4) 51956
n-synaptobrevin-QF2 3rd (att86Fb) 51955
n-synaptobrevin-QF2w 3rd (attP2; 68A4) 51960
n-synaptobrevin-GAL4:QF 3rd (attP2; 68A4) 51945
n-synaptobrevin-LexA:QF 3rd (attP2; 68A4) 51953
   
tubulin-QF2 2nd 51958
tubulin-QF2 3rd 51957
tubulin-QF2w 2nd 51962
tubulin-QF2w 3rd 51963
actin-GAL4:QF 2nd 59285
actin-QF2w 2nd 51961
GMR-QF2w 2nd 59283
Additional QF stocks can be found on the Q-system Bloomington Drosophila Stock Center Website

QS lines

Name Chromosome Bloomington Stock #
tubP-QS X Available Upon Request
tubP-QS #L1 2L (30C5) 30024
tubP-QS 2R Available Upon Request
tubP-QS #A 3L (78C8) 30021
tubP-QS #9B 3R (100E1) 30022
DH/ TM6B, tubP-QS #4A TM6B balancer 30023

UAS reporters

Name Chromosome Bloomington Stock #
   
UAS-QS 3rd (attP2) 30033
   
UAS>stop>mCD8-GFP #11 2nd 30125
UAS>stop>mCD8-GFP #14, UAS>stop>mCD8-GFP#18 3rd 30032
   
UAS-mtdTomato-3xHA 3rd (ZH-attP-86Fa) 300124

Recombinant Animals

Name Chromosome Bloomington Stock #
   
GH146-QF recombinants:  
GH146-QF#53, QUAS-mCD8-GFP 3rd 30038
GH146-QF#53, QUAS-mtdT-3xHA 3rd 30037
GH146-QF#53, 82BFRT, tubP-QS#9B 3rd 30039
   
ET40-QF recombinants:  
ET40-QF, QUAS-mtdT-3xHA #14 2nd 30043
actin-GAL4:QF, UAS-mtdT-3xHA 2nd 59284

Stocks for MARCM analysis

Name Chromosome Bloomington Stock #
   
Q-MARCM  
tubP-QS #3A, 19AFRT X (3F9) 30129
tubP-QS #O, 19AFRT X (13F1) 30130
tubP-QS #4C, 40AFRT 2L (35D4) 30035
tubP-QS #J, 40AFRT 2L (35B2) 30131
G13FRT ,tubP-QS #6 2R (47A7) 30132
G13FRT ,tubP-QS #5A 2R (59F1) 30133
tubP-QS #F2, 80BFRT 3L (77B9) 30537
82BFRT, tubP-QS#9B 3R (100E1) 30034
82BFRT, tubP-QS#21 3R (95A7) 30117
   
GAL4 MARCM  
tubP-GAL4, 82BFRT/TM6B 3rd (79A2) 30029
tubP-GAL4, 82BFRT, tubP-GAL80/TM6B 3rd 30036
tubP-GAL4, 82BFRT, tubP-GAL80, Tsc1Q600X /TM6B 3rd Available Upon Request
y, w; ET40-QF, QUAS-mtdT-3xHA; 82BFRT, tubP-QS#9 2nd, 3rd 30042
   
hsFLP, QUAS-mtdT-3xHA, UAS-mCD8-GFP X 30118
QUAS-mCD8-GFP#5B, tubP-GAL4/TM6B 3rd 30031

GAL4 system reagents

Name Chromosome Bloomington Stock #
GAL4 reagents  
acj6-GAL4/FM7c X 30025
GH146-GAL4 2nd 30026
NP21-GAL4 3rd 30027
acj6-GAL4, UAS-mCD8-GFP/FM7c; Pin/CyO X 30028
tubP-GAL4, UAS-mCD8-GFP 3rd 30030
nsyb-GAL4 (attP2) 3rd 51941
nsyb-GAL4 (86Fb) 3rd 51942
nsyb-GAL4 (attP2), UAS-nucLacZ 3rd 51943
nsyb-GAL4 (attP2), UAS-mCD8GFP 3rd 51944
   
GAL80 enhancer trap  
ET-GAL80, y+ #1 X (GAL80 enhancer trap) 30040
ET-GAL80, y+ #2 2nd (GAL80 enhancer trap) 30041