Q-system reagents - DNA

QF constructs

Name Description
pattB-QF-hsp70 QF sequence with hsp70 terminator, in attB PhiC31 integrase vector. This vector is recommended for cloning of QF constructs.

Map  Sequence

pattB-QF QF ORF with SV40 terminator. Leads to higher expression of QF.

Map  Sequence

pattB-DSCP-QF QF ORF with DSCP promoter and SV40 terminator.

Map  Sequence

pBac-GH146-QF piggyBac vector containing GH146 enhancer element driving QF. Marked by 3xP-DsRed

Map  Sequence

pQF-ET QF-sv40 enhancer trap.

Map  Sequence

pQF-M2ET QF-sv40 enhancer trap flanked by LoxM2 and LoxP sites, compatible with RCME.

Map  Sequence

pCMV-QF Mammalian QF tissue culture expression vector. CMV promoter driving QF.

Map  Sequence

pAC-QF Drosophila QF tissue culture expression vector. Actin5C promoter driving QF.

Map  Sequence

   
pattB-nsynaptobrevin-QF2 pattB vector with n-syb promoter expressing QF2 Addgene #46115
pattB-nsynaptobrevin-QF2w pattB vector with n-syb promoter expressing QF2w Addgene #46116
pattB-nsynaptobrevin-GAL4:QF pattB vector with n-syb promoter expressing GAL4 DNA binding domain with QF activation domain Addgene #46113
pattB-nsynaptobrevin-Lex:QF pattB vector with n-syb promoter expressing LexA DNA binding domain with QF activation domain Addgene #46123
   
Additional QF2 reagents can be found at the following link: New QF2 reagents
   
  Email cpotter@jhmi.edu for additional information  
   

QS constructs

Name
Description
pBS-SK-QS QS ORF cloned into pBluescript vector. Recommended for cloning of QS ORF.

Map  Sequence

ptubP-QS pCasper4 vector containing tubulin promoter driving QS.

Map  Sequence

pUASTattB-QS PhiC31 integrase attB vector containing UAS-QS

Map  Sequence

CMV-QS Mammalian QS tissue culture expression vector. CMV promoter driving QS.

Map  Sequence

pAC-QS Drosophila QS tissue culture expression vector. Actin5C promoter driving QS.

Map  Sequence

QUAS constructs

Name
Description
pQUAST QUAST P-element transformation vector for cloning of QUAS-geneX constructs.

Map  Sequence

pQUAST-mCD8-GFP Membrane targeted GFP expression vector.

Map  Sequence

pQUAST-mtdT-3xHA Membrane targeted tandom Tomato red fluorescent expression vector with 3x C-terminal HA tag.

Map  Sequence

pQUAST-nucLacZ Nuclear targeted beta-galactosidase expression vector.

Map  Sequence

pQUAST-shibirets1 Temperature sensitive dynamin expression vector.

Map  Sequence

pQUAST-FLPo Mammalian optimized FLPase expression vector.

Map  Sequence

pQUAST-DSCP+FLPo Mammaliant optimized FLPase expression vector with DSCP promoter.

Map  Sequence

pCa4B2G-QUAS-FLPo Mammalian optimized QUAS-FLPase flanked by gypsy insulators.

Map  Sequence

pQUAST-FRT-STOP-FRT-mCD8GFP Intersectional vector. QUAS followed by FRT site, two transcriptional terminators, FRT site, and membrane targeted GFP.

Map  Sequence

pQUAST-FRT-STOP-FRT-SHIBIRETS1 Intersectional vector. QUAS followed by FRT site, two transcriptional terminators, FRT site, and temperature sensitive dynamin.

Map  Sequence

pQUAS-GG Mammalian tissue culture expression vector. QUAS driving GFP expression.

Map  Sequence

QUAS-luc2 Tissue culture expression vector. QUAS driving firefly luciferase expression.

Map  Sequence

Other

Name
Description
pAC-GAL4 Drosophila tissue culture expression vector. Actin5c promoter driving GAL4 expression.

Map  Sequence

pAC-GAL80 Drosophila tissue culture expression vector. Actin5c promoter driving GAL80 expression.

Map  Sequence

pAC-hRluc Drosophila tissue culture expression vector. Actin5c promoter driving Renilla luciferase expression.

Map  Sequence

pCMV-GAL4 Mammalian tissue culture expression vector. CMV promoter driving GAL4 expression

Map  Sequence

pCMV-GAL80 Mammalian tissue culture expression vector. CMV promoter driving GAL80 expression

Map  Sequence

     
pUAS-GG Tissue culture expression vector. UAS promoter driving GFP expression

Map  Sequence

UAS-luc2 Tissue culture expression vector. UAS promoter driving luciferase expression

Map  Sequence

     
pUAST-FRT-stop-FRT-mCD8GFP Intersectional vector. UAS promoter followed by FRT site, two transcriptional terminators, FRT site, and membrane targeted GFP.

Map  Sequence

pUASTattB-mtdT-3xHA PhiC31 integrase UASattB vector containing membrane targeted tandom tomato with 3x C-terminal HA tag.

Map  Sequence

     
pmCD8-GFPy+ mCD8-GFP enhancer trap, marked by yellow transgene.

Map  Sequence